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. 2022 Mar 8;12:4043. doi: 10.1038/s41598-022-08049-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN overexpression in JURKAT cells. (A) The effect of inducible PTEN WT and PTEN G129R expression on the sensitivity of JURKAT cells to ASNase based on MTS assays. (B) The immunoblot analysis of PTEN, P-Akt (Ser473), P-Akt (Thr308), P-S6, S6, P-GSK-3β and GSK-3β in JURKAT cells with inducible PTEN WT (W) and PTEN G129R (M) expression, ALL-SIL and DND-41 cell lines. β-Actin is shown as a loading control. (C) The sensitivity of JURKAT cells transduced with pHIV-PTEN or EV to ASNase based on MTS assays. (D) The glycolytic profile of JURKAT cells transduced with pHIV-PTEN or EV was determined using Seahorse XFp analyzer and Glycolysis Stress Test. (E) The immunoblot analysis of P-Akt (Ser473), P-Akt (Thr308), P-S6 and S6 in JURKAT cells transduced with pHIV-PTEN and EV. GAPDH is shown as a loading control. The MTS assays were done at least in biological triplicates and six technical replicates. * = FDR < 0.1%. The Seahorse measurements were done at least in biological triplicates and six technical replicates. All the results are presented as a mean ± SD. Each immunoblot is a representative result of three independent experiments. Different gels are separated by lines.