Table 1.

Sequencing and diagnostic parameters of the investigated fox samples.

Accession number	Season	Age Category	RT-qPCR Ct value	Number of multiplex PCR cycles	Processed and mapped reads	Mean coverage on the targeted region (reads)
OK557779	Summer	Cub	21.50	25	19,846	1868.1
OK557780	Summer	Cub	24.15	25	14,192	1347.4
OK557781	Summer	Juvenile	29.25	28	14,244	1398.3
OK557782	Summer	Cub	26.02	28	14,491	1231.2
OK557783	Summer	Juvenile	27.12	28	12,588	1090.2
OK557784	Summer	Juvenile	25.46	28	6513	579.0
OK557785	Summer	Adult	36.90	36	14,186	1263.0
OK557786	Summer	Juvenile	27.83	28	11,827	1011.4
OK557787	Summer	Juvenile	38.58	36	10,750	1102.3
OK557788	Summer	Juvenile	28.15	28	8871	810.3
OK557789	Summer	Adult	38.25	36	4485	320.2
OK557790	Summer	Juvenile	28.32	28	10,203	1114.6
OK557791	Summer	Juvenile	26.74	28	8494	801.7
OK557792	Summer	Juvenile	22.83	25	7461	674.2
OK557793	Summer	Juvenile	21.49	25	12,266	1232.6
OK557794	Spring	Cub	31.33	32	93,228	5662.0
OK557795	Spring	Cub	31.91	33	63,959	3984.3
OK557796	Spring	Cub	30.78	32	56,054	3460.9
OK557797	Spring	Adult	32.69	33	80,721	4696.6

Most relevant next-generation sequencing quality data as the mapped reads and mean coverage per sample is presented. Number of multiplex PCR cycles is relevant for the amplicon-based NGS sequencing workflow.