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. 2022 Mar 8;12:4042. doi: 10.1038/s41598-022-08053-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in OD at 540 nm (ordinate) represent the results obtained from the plasma-free hemoglobin spectrophotometric assay that indicated erythrocyte breakdown with time of blood incubation in the experimental system (MANOVA test results for interaction, time, and group were p < 0.001, p < 0.001, p < 0.05, respectively) (A). The results are presented as mean values ± standard deviation. **p < 0.01 for CTR (empty bar) vs NIR 1.5 mW/cm² (diagonal pattern) at a given time point. Furthermore, significant changes (***p < 0.001) were also found compared to other time points within each group, and different time points for intergroup comparison. The number of measurements n = 20. Differences in the parameters of the hemolysis curve at t (4 h): osmotic fragility (B) and the slope of the hemolysis curve (C) (Wilcoxon’s test in both cases). The results are presented as the median value and the interquartile range. The number of measurements n = 10.