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. 2022 Mar 8;13:1217. doi: 10.1038/s41467-022-28872-x

Fig. 3. RBV promotes the transcription of MIR genes.

Fig. 3

a Three-week-old plants of the indicated genotypes. Bar = 1 cm. b Levels of seven pri-miRNAs in 14-day-old seedlings of rbv-1 and the complementation line pRBV:RBV-eYFP rbv-1 as determined by RT-qPCR. UBQ5 was used as the internal control. Error bars represent standard deviation calculated from three independent replicates. (Student’s t test, **P < 0.01). c Representative images of GUS staining of pMIR167a:GUS and pMIR167a:GUS rbv-1 inflorescences. Bars = 2 mm. d Transcript levels of GUS from the two genotypes as determined by RT-qPCR. The expression values were relative to pMIR167a:GUS. Error bars represent standard deviation calculated from three independent replicates. (two-tailed Student’s t test, *P < 0.05). e RBV is required for the recruitment of Pol II to MIR166a and MIR167a promoters. The occupancy of Pol II at various regions was determined by ChIP with rbv-1 and Col using an antibody that recognizes the C-terminal repeat (YSPTSPS) of the largest subunit of Pol II. ChIP performed without the antibody served as a negative control. A genomic region between the genes AT2G17460 and AT2G17470 named Pol II C1 was also used as a negative control. Mean and standard deviation from three independent replicates are presented. (Student’s t test, **P < 0.01). Source data are provided as a Source Data file.