. 2022 Feb 23;10:824812. doi: 10.3389/fcell.2022.824812

TABLE 1.

Osteogenic differentiation potential different MSC subpopulations.

MSC subpopulations	Control	Species	Analysis methods	Results	References
BMSCs transfected with CD200	BMSCs transfected without interposed gene	human	ALP staining and gene expression and protein production of Runx2	CD200 expression increased the levels of ALP activity and Runx2 expression in BMSCs	Kim et al. (2018a)
CD73⁺ BMSCs	CD73⁻ BMSCs	mouse	Alizarin red staining, bone fracture repair in vivo	CD73⁺ BMSCs exhibited enhanced potentials for osteogenic differentiation in vitro and fracture repair in vivo	Kimura et al. (2021)
CD73⁺ CD39⁺	CD73⁺ CD39⁻ SMSCs	human	Alizarin red staining and expression of osteoblast genes	CD73⁺ CD39⁺	Gullo and De Bari, (2013)
SMSCs	CD73⁺ CD39⁻ SMSCs	human	Alizarin red staining and expression of osteoblast genes	SMSCs showed increase in calcium accumulation and gene expression of Runx2	Gullo and De Bari, (2013)
CD10^High ADSCs	ADSCs	human	Alizarin red staining	CD10^High ADSCs exhibited higher level of calcium accumulation	Kouroupis et al. (2020)
CD271⁺ BMSCs	PA BMSCs	human	Alizarin red S staining	CD271⁺ BMSCs had a larger mineralized area	Quirici et al. (2002)
CD271⁺ ADSCs	CD271^- ADSCs	mouse	Alizarin red S staining	CD271⁺ ADSCs were more prone to form calcium nodule after osteogenic differentiation	Yamamoto et al. (2007)
CD271⁺ ADSCs	CD271^- ADSCs	human	Alizarin red S staining		Barilani et al. (2018)
CD271⁺ DPSCs	CD271^- DPSCs	human	ALP staining, Ca²⁺ level, and genes expression of Runx2, Osterix, Osteocalcin, and Nestin	ALP activity and Ca²⁺ levels were lower in CD271⁺ DPSCs; no difference in the expression level of osteogenic genes was detected	Mikami et al. (2011)
CD146⁺ PDSCs	CD146^- PDSCs	human	von Kossa staining	CD146⁺ PDSCs exhibited a higher level of spontaneous ossification	Ulrich et al. (2015)
CD146^Low PCy-MSCs	CD146^High PCy-MSCs	human	Alizarin red staining and expression of osteoblast genes	calcium accumulation and genes expression of Runx2 and Osteopontin were greater in the CD146^Low than in CD146^High PCy-MSCs	Paduano et al. (2016)

BMSCs, bone marrow-derived mesenchymal stem cells; ALP, alkaline phosphatase; SMSCs, synovial membrane-derived mesenchymal stem cells; ADSCs, adipose tissue-derived mesenchymal stem cells, PA, plastic adherent; DPSCs, dental pulp-derived mesenchymal stem cells; PDSCs, placenta-derived mesenchymal stem cells; PCy-MSCs, periapical cyst mesenchymal stem cells.