FIGURE 1.

Vav1 protein deficiency affects maturation of recirculating B cells in the BM. Statistical analyses of flow cytometry representing total BM cells (A), total B cells (B), representative flow cytometry plots of IgM/B220 B cell subpopulations of each genotype (C), statistical analyses of flow cytometry representing percentages of pro/pre B (B220^low IgM⁻) (D), immature (B220^low IgM⁺) (E), transitional (B220^low IgM^high) (F), representative flow cytometry plots of recirculating B cells for each genotype (G) and percentages of recirculating (B220^high IgM⁺) (H) B cells. Each point represents data from a single mouse. Data in the graphs are shown as means ± SD (n = at least 4 mice per group). Data are merged from at least three independent experiments. *p < 0.05, and **p < 0.01. p-values were determined using a two-tailed Student’s t test or Mann-Whitney-U test. WT, Vav1-, Vav3-and Btk/Vav3-knockouts were analyzed in an age range from 7–15 and Btk- and Btk/Vav3-knockouts from 8–29 weeks (3 mice each were analyzed in the age range from 26–29 weeks, remaining animals were 8–14 weeks old). Gating strategy is shown in Supplementary Figure S4.