FIGURE 2.

Btk/Vav double deficiency causes reduced B cell numbers in the blood. (A) Numbers of total WBCs measured with an Animal Blood Counter are shown. Statistical analyses of flow cytometry representing total CD19⁺ B cells (B), representative flow cytometry plots of IgM/IgD B cell subpopulations in the blood for each genotype (C), statistical analyses of flow cytometry representing IgM^high IgD^low (D), IgM^high IgD^high (E) and IgM^low IgD^high (F) B cell percentages in the blood are shown. Each point represents data from a single mouse. Data in the graphs are shown as means ± SD (n = at least 4 mice per group). Data are merged from at least three independent experiments. *p < 0.05, and **p < 0.01. p-values were determined using a two-tailed Student’s t test or Mann-Whitney-U test. WT, Vav1-, Vav3-and Btk/Vav3-knockouts were analyzed in an age range from 7–15 and Btk- and Btk/Vav3-knockouts from 8–29 weeks (3 mice each were analyzed in the age range from 26–29 weeks, remaining animals were 8–14 weeks old). Gating strategy is shown in Supplementary Figure S5.