FIGURE 3.

Vav protein deficiency affects B cell maturation in the spleen. Statistics of flow cytometry representing splenocytes (A), total B220⁺ B cells (B), representative flow cytometry plots of splenic IgM/IgD B cell subpopulations of each genotype (C), statistics of flow cytometry representing percentage of IgM^high IgD^low (D), IgM^high IgD^high (E) and IgM^low IgD^high (F) B cells in the spleen. Representative flow cytometry plots of splenic T0, T1 and T2 stage B cell subpopulations of each genotype (G), statistics of flow cytometry representing percentage of T0 stage (H), T1 stage (I) and T2 stage (J) B cells. Representative flow cytometry plots (K) and statistics (L) of MZ B cells. Each point represents data from a single mouse. Data in the graphs are shown as means ± SD (n = at least 6 mice per group). Data are merged from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p-values were determined using a two-tailed Student’s t test or Mann-Whitney-U test. Mice were analyzed in an age range from 10–16 weeks. Gating strategy is shown in Supplementary Figure S6.