FIGURE 4.

Vav protein deficiency causes disturbed splenic microarchitecture. (A) Representative HE-stainings of splenic sections Scale bar indicates 200 µm. (B, C) Statistical analyses showing number of follicles (B) and follicle size (C) determined within a certain section. Three sections were analyzed per animal. Each point represents one analyzed image section. Data in the graphs are shown as means ± SD. Spleens of WT (n = 12), Btk-ko (n = 9), Vav1-ko (n = 5), Btk/Vav1-ko (n = 13), Vav3-ko (n = 15) and Btk/Vav3-ko (n = 9) animals were stained and analyzed. Data are merged from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p-values were determined using a two-tailed Student’s t test or Mann-Whitney-U test. Mice were analyzed in an age range from 9–13 weeks