FIGURE 7.

Vav protein deficiency impairs B cell differentiation into ASCs in vitro. Naïve B cells were isolated from respective mouse strains and cultured in the presence of CD40 and IL4 for 7 days. (A. B) On day 4, cells were collected and analyzed for the presence of CD138⁺ B220^low ASCs by flow cytometry. Representative flow cytometry plots for each genotype (A) and statistical analysis (B) are shown. (C) On day 7, culture supernatants were collected and secreted amounts of IgM and IgG1 are shown. Data are merged from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. p-values were determined using a two-tailed Student’s t test or Mann-Whitney-U test. Mice were analyzed in an age range from 10–13 weeks