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. 2022 Mar 9;1203:339702. doi: 10.1016/j.aca.2022.339702

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© 2022 Published by Elsevier B.V.

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Fig. 3 — LOD of SARS-CoV-2 detection on chip. (A) PD-LAMP diffusion coefficient measurements show a decreasing trend with higher concentration of virus (from 5 × 10³ to 5 × 10¹ virus particles (20 μL reaction) ⁻¹). A significant difference from the NTC was seen for 5 × 10¹, 5 × 10² and 5 × 10³ (∗∗∗∗p < 0.0001) virus particles per reaction using one-way ANOVA with Dunnett's post-hoc relative to NTC (N = 3). (B) A representative image of 2% agarose gel confirming amplification in the positive samples as seen by the LAMP banding pattern.