Fig. 5.

On chip heating sensitivity and specificity of PD-LAMP for SARS-CoV-2 samples containing 8% and 10% saliva. MERS and SARS inactivated virus were diluted to a concentration of 1 × 10⁴ particles μL⁻¹. SARS-CoV-2 inactivated virus was diluted with DEPC treated nuclease free water at concentrations ranging from 5 × 10³ to 5 × 10¹ virus particles per 20 μL reaction in 10-fold dilutions. (A) Diffusion coefficient measurements from reactions containing 8% saliva indicate significant differences from the NTC for 5 × 10¹, 5 × 10² (∗∗p < 0.01) and 5 × 10³ (∗∗∗∗p < 0.0001) virus particles per 20 μL reaction. The same significance was found against MERS and then SARS. There was no significant difference between the negative controls. (B) Diffusion coefficient measurements from reactions containing 10% saliva indicate significant differences from the NTC, MERS and SARS for 5 × 10¹, 5 × 10², and 5 × 10³ (∗∗∗∗p < 0.001). There was no significant difference between NTC and SARS or NTC and MERS. However, MERS was significantly different from SARS (∗p < 0.05). Statistical analysis was performed with Tukey's multiple comparison test (N = 3).