Fig. 6.

Performing PD-LAMP on-chip with lyophilized reagents. MERS and SARS inactivated virus were added at a concentration of 1.6 × 10⁴ virus particles per 20 μL reaction. SARS-CoV-2 inactivated virus was diluted with DEPC treated nuclease free water at concentrations ranging from 5 × 10³ to 5 × 10¹ viral particles per reaction in 10-fold dilutions. Diffusion coefficient measurements from reactions containing 8% saliva indicate significant differences from the controls (NTC, MERS, and SARS) for 5 × 10¹, 5 × 10² (∗∗p < 0.01) and 5 × 10³ (∗∗∗∗p < 0.0001) virus particles per 20 μL reaction. Statistical analysis was performed using Tukey's multiple comparison test (N = 3).