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. 2022 Jan 1;27:699–717. doi: 10.1016/j.omtn.2021.12.035

Figure 5.

Figure 5

The inhibitory effects of FTO on GBM progression are reversed by miR-10a

(A) Analyses of the correlation between the expression of FTO and MTMR3, a miR-10a target, in five datasets. Statistical analysis was determined using Pearson correlation coefficient analyses. (B) MTMR3 mRNA expression in GBM cells transduced with FTO overexpression, knockdown, and the corresponding control lentiviruses. (C) Protein expression of MTMR3, CD44, PCNA, FTO, and GAPDH in U87MG cells co-transduced with ovNC or ovFTO lentivirus and mimics NC or miR-10a-5p mimics as indicated. Quantification histogram represents relative protein expression. (D) LN229 cells were co-transduced with shNC, shFTO-1, or shFTO-2 lentivirus and inhibitor NC or miR-10a-5p inhibitor as indicated. Quantification histogram represents relative protein expression. RNA expression of (E) miR-10a-5p and (F) MTMR3 in U87MG cells co-transduced with ovNC or ovFTO lentivirus and mimics NC or miR-10a-5p mimics as indicated. LN229 cells were co-transduced with shNC, shFTO-1, or shFTO-2 lentivirus and inhibitor NC or miR-10a-5p inhibitor as indicated. (G) CCK-8 assay of U87MG cells transfected with (left) mimics NC or miR-10a-5p mimics in the presence or absence of FTO overexpression of LN229 cells transfected with inhibitor NC or (right) miR-10a-5p inhibitor in the presence or absence of FTO knockdown. (H) Transwell assay of U87MG cells transfected with mimics NC or miR-10a-5p mimics in the presence or absence of FTO overexpression as indicated. Scale bar, 50 μm. (I) Transwell assay of LN229 cells transfected with inhibitor NC or miR-10a-5p inhibitor in the presence or absence of FTO knockdown. Scale bar, 50 μm. (J) IHC of MTMR3 expression in U87MG cells in xenograft sections of nude mice. Scale bar, 50 μm. Comparisons between two independent samples and among multiple samples were performed using two-tailed t tests and one-way ANOVA, respectively. Error bars indicate at least three independent experiments, and data are shown as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.