Figure 4.

Anti-arthritis effects of miR-10b inhibitor on CIA

Mice immunized with CII/CFA were randomly divided into three groups and administered miR-10b antagomir or antagomir NC FAM by tail intravenous administration at the indicated doses every 3 days since the grouping. (A) The time indicates the of induction of the CIA model and administration of miR-10b antagomir or antagomir NC FAM. (B) The arthritis index and (C) the swollen joint count of individual CIA mice from day 29 to day 59 after the first immunization. (D) The representative photographs of paws and effects of miR-10b antagomir. The effects of miR-10b inhibitor on the expression of miR-10b in (E) PBMCs and (F) synovium of mice. (G) The arthritis index and the swollen joint count (H) were evaluated, as were the effects of miR-10b antagomir. The thickness of (I) front paws and (J) hind paws of mice were measured. (K) The bodyweight of CIA mice was restored by miR-10b antagomir. Data are presented as mean ± SD from three independent experiments with at least five mice pre group. Two-way ANOVA followed by multiple comparison test; ∗∗∗p < 0.001 versus normal group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus antagomir NC FAM; ns, no significance.