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. 2021 Oct 4;35(12):917–950. doi: 10.1089/ars.2020.8234

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© László Potor et al., 2021; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License [CC-BY-NC] (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 12. — Internalization of ferryl hemoglobin via CD163 receptor. (A, B) Macrophages were grown on coverslips and were exposed to ferrylHb (10 μM; second row) in the presence or absence of siRNA against CD163 receptor (200 nM; third row) or with—siRNA (200 nM; fourth row) for 16 h. Cells were stained with Hoechst 33258 for DNA (blue), an anti-ferrylHb antibody with Alexa Fluor 488 secondary antibody for ferrylHb (green), and an anti-CD163 antibody with Alexa Fluor 647 antibody for CD163 (red). (A) Low-magnification and (B) high-magnification images were shown. Images were taken with Leica TCS SP8 gated STED-CW nanoscopy. Images were deconvolved using Huygens Professional software. (A, lower panel) FerrylHb intensity of macrophages was calculated by ImageJ software. (B, lower panel) CD163 intensity of macrophages was calculated by ImageJ software. Scale bars shown in the images represent (B) 5 μm and (A) 25 μm. **p < 0.01; ***p < 0.001. siRNA, small interfering RNA. Color images are available online.