FIG. 13.

Transcriptomic analysis reveals that ferryl hemoglobin polarizes macrophages into a proinflammatory-like phenotype. (A, B) RNA-seq transcriptomic analysis was performed on human macrophages treated with ferrylHb (10 μM) for 8 h (n = 4/group). (A) Heatmap of the 50 most-changed DE genes is shown in log₁₀(FC). (B) M1 and M2 signatures are shown as a clustered heatmap in log₁₀(FC). (C, D) Relative expression of (C) IL-1β, TNF-α, CXCL8, (D) CD209, and IL-27RA were analyzed by real-time qPCR (n = 3). (E) Protein expression of HO-1, H-ferritin, Pro-IL-1β, IL-1β, TNF-α and GAPDH was determined by Western blots in macrophages exposed to ferrylHb (10 μM, 8-h ferrylHb treatment). **p < 0.01; ***p < 0.001. DE, differentially expressed; FC, fold change. Color images are available online.