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Growth and SigF specific expression curves of M. bovis BCG containing pCK3215 in liquid culture. Aliquots were removed daily to measure the optical density; the numbers of CFU and amount of β-galactosidase (βgal) activity. Solid lines represent the optical density at 600 nm, and dashed lines represent relative units of β-galactosidase activity per CFU. Relative units of β-galactosidase activity/CFU = actual units of β-galactosidase activity/CFU × 1010.
