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. 2022 Feb 23;9:837143. doi: 10.3389/fmed.2022.837143

Figure 5.

Figure 5

Inhibition of TKT induces cell apoptosis. (A,B) HCT116 cells were treated with DMSO or the TKT inhibitor N3PT (10 μM) for 24 h. Cell apoptosis was assayed by co-staining with Annexin V/PI. There is no significant difference in the apoptotic cell ratio between the 10 uM N3PT treated group and the control group. (C,D) HCT116 cells were transfected with TKT siRNA or negative control (NC siRNA) for 72 h. Cell apoptosis was assayed by co-staining with Annexin V/PI. The number of both early apoptotic and late apoptotic cells was significantly increased in the TKT siRNA group (C). The apoptotic cell ratio was also significantly increased in the TKT siRNA group compared with the NC siRNA group (D). Apoptotic cells are presented as the mean ± SD of three independent experiments (lower left: live cells, lower right: early apoptotic cells, upper right: late apoptotic cells, upper left: dead cells). N = 3/group, ***P <0.001 vs. the control group.