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. 2022 Feb 23;9:837143. doi: 10.3389/fmed.2022.837143

Figure 7.

Figure 7

Inhibition of TKT blocks Notch signaling pathway. (A–D) HCT116 cells were treated with DMSO or the TKT inhibitor N3PT (1, 5, or 10 μM) for 24 h. Then the protein levels in cell lysates were quantified by Western blot (A). The quantized data indicated that 10 μM N3PT treatment reduced the protein levels of NICD (B), Hes1 (C), and TKT (D) compared with the control. However, lower concentrations of N3PT had no significant effect on the protein levels of NICD, Hes1, and TKT (A–D). (E,F) HCT116 cells were transfected with TKT siRNA or negative control (NC siRNA) for 72 h. Then the protein levels in cell lysates were quantified by Western blot (E). Cells transfected with TKT siRNA presented lower protein levels of NICD (F), Hes1 (G), and TKT (H) than NC siRNA transfected cells. N = 3/group, *P <0.05, **P <0.01, ***P <0.001 vs. the control group.