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. 2022 Feb 23;13:827523. doi: 10.3389/fendo.2022.827523

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Copyright © 2022 Zhou, Xu, Wang, Ling, Nong, Xie, Zhu and Shan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cold exposure changed the transcriptome profile of VAT in pigs. (A) Volcano plot of differently expressed genes (DEGs) expression patterns were illustrated. Red denotes upregulated genes in COLD VAT; blue denotes downregulated genes in COLD VAT; gray denotes genes with no significant changes. (B) Gene Ontology (GO) enrichment analysis. (C) Functional enrichment analyses using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. (D, E) Heatmaps of the TPM expression values of selected thermogenesis, fatty acid metabolism regulated genes from the RNA-seq dataset. (F–H) KEGG results of the cold-induced enrichment of genes involved in TGF-beta signaling pathway (F), FoxO signaling pathway (G), PPAR signaling pathway (H).