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. 2022 Feb 23;13:827523. doi: 10.3389/fendo.2022.827523

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Copyright © 2022 Zhou, Xu, Wang, Ling, Nong, Xie, Zhu and Shan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cold exposure induced alterations in heat production processes. (A–D) qPCR validation of the expression of genes related to thermogenesis and fatty acid metabolism in SAT and VAT from RT and cold-treated pigs (n = 5). (E, F) Transcripts per million (TPM) expression values of lipolysis markers (ATGL, HSL) and cold-induced genes (UCP2, UCP3) of SAT and VAT from cold-treated and RT pigs were shown to estimate relative gene expression abundance (n = 3). Error bars represent SEM. *P < 0.05, **P < 0.01, two-tailed Student’s t-test.