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. 2022 Feb 23;12:761604. doi: 10.3389/fcimb.2022.761604

Figure 1.

Figure 1

Roles of hcp gene and iron ions in ATCC17978, ATCC17978Δhcp, and ATCC17978Δhcp+ biofilm formation. (A) Biofilm formation in 24-well cell culture plates. (B) Crystal violet staining of biofilm formation rings in 5-ml polystyrene tubes. (C) Ratio of optical density (OD) of biofilm formation measured by crystal violet staining and growth turbidity (OD580/OD600) (the asterisks indicate a significant difference in biofilm formation ability between ATCC17978 and ATCC17978Δhcp strains at the same iron ion concentration). *** p < 0.0001.