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. 2022 Feb 23;12:839880. doi: 10.3389/fonc.2022.839880

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Copyright © 2022 Giusti, Di Francesco, Poppa, Esposito, D’Ascenzo and Dolo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of EVs_30′ and EVs_18h on fibroblasts’ proliferation. Proliferation was evaluated by XTT assay, on the 5th day of treatment, i.e., at 96 h from the beginning of the EV treatment. Values were calculated as mean ± SD and are expressed as percentages with respect to 100% proliferation, conventionally attributed to untreated NHDF. Experiments were performed three times in triplicate. The asterisk on the bar indicates the statistical significance with respect to NHDF, and the horizontal line refers to the statistical significance between the NHDF_30′ and NHDF_18h (*p < 0.05).