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. 2022 Feb 23;12:839880. doi: 10.3389/fonc.2022.839880

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Copyright © 2022 Giusti, Di Francesco, Poppa, Esposito, D’Ascenzo and Dolo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning electron microscopy (SEM). SEM images highlighted the intense shedding of microvesicles from the plasma membrane of NHDF_30′ and NHDF_18h. On the other hand, NHDF cells showed an extremely sporadic production of microvesicles. The first row shows images at low magnification; the second row shows details of the first row at higher magnification, as highlighted by the boxes; row 3 shows other independent and representative images at higher magnification. The scale bar is 10 µm in all images.