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. 2022 Feb 23;12:839880. doi: 10.3389/fonc.2022.839880

Figure 8.

Figure 8

Effect of NHDF, NHDF30′, and NHDF18h secretome on CABA I cells. (A) CABA I cells were cultured for 72 h with conditioned media (CMs) of NHDF, NHDF30′, and NHDF18h. Data, derived from experiments performed twice in triplicates, are expressed as mean ± SD and shown as a percentage, and 100% proliferation was assigned to CABA I cells proliferating with CM of untreated NHDF. (B) CABA I cell migration was measured twice in duplicate in response to serum-free CM NHDF, CM NHDF30′, and CM NHDF18h, by modified Boyden chamber (*p < 0.05). Data (mean ± SD) are expressed as a percentage with respect to 100% migration, conventionally attributed to CABA I cells migrating toward CM NHDF. (C) CABA I cell invasion was tested twice in response to CM NHDF, CM NHDF30′, and CM NHDF18h with a modified Boyden chamber coated with Matrigel® (*p < 0.05). Data (mean ± SD) are expressed as a percentage, and 100% invasion was attributed to CABA I cells migrating toward CM NHDF.