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. 2022 Feb 23;12:839880. doi: 10.3389/fonc.2022.839880

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Copyright © 2022 Giusti, Di Francesco, Poppa, Esposito, D’Ascenzo and Dolo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of NHDF, NHDF_30′, and NHDF_18h secretome on HUVECs. (A) The effect of conditioned media (CM) NHDF, CM NHDF_30′, and CM NHDF_18h on endothelial cell growth was assessed using the XTT assay. Proliferation was expressed as a percentage, conventionally attributing 100% proliferation to HUVECs treated with CM NHDF. Data derived from experiments performed twice in triplicates. (B) Representative pictures showing the formation of capillary-like structures formed by HUVECs seeded on Matrigel^®-coated plates in a serum-free condition and treated with CM NHDF, CM NHDF_30′, and CM NHDF_18h; the graphs at the bottom show the number of nodes or total length of tubes or number of segments normalized per area. Data derive from experiments performed twice in duplicate and are expressed as the mean ± SD (*p < 0.05; **p < 0.01).