TABLE 1.
Bacterial strains and plasmids used in this study
| S. aureus strain derived from NCTC 8325 or plasmid | Relevant genotype or insert | Relevant phenotype | Origin or reference |
|---|---|---|---|
| Strains | |||
| BB255 | Wild type | 3 | |
| BB270 | Mcr | 3 | |
| PG27 | Ω12FglmM::Tn551 | Mcs Emr; small colonies | This study; by transduction of Ω12FfemD::Tn551 from BB591(4) into BB255 |
| PG108 | mec Ω12FglmM::Tn551 | Mcs Emr | This study; by transduction of Ω12FfemD::Tn551 from BB591(4) into BB270 |
| PG100 | mec Ω12FglmM::Tn551 hmrD1 | Mcr Emr | This study; Mcr suppressor mutant of PG108 |
| PG105 | mec Ω12FglmM::Tn551 | Mcs Emr | This study; backcross of Emr from PG100 into BB270 |
| PG79 | mec Ω12FglmM::Tn551; pPG76 | Mcr Emr Tcr | This study; by transduction of PG108 |
| PG217 | mec Ω12FglmM::Tn551; pAW8 | Mcs Emr Tcr | This study; by transduction of PG108 |
| RN4220 | NCTC 8325-4 r− m+ | Restriction negative, modification positive | 21 |
| PG77 | NCTC 8325-4 r− m+; pAW8 | Tcr | This study; by electroporation of RN4220 |
| PG78 | NCTC 8325-4 r− m+; pPG76 | Tcr | This study; by electroporation of RN4220 |
| Plasmids | |||
| pAW8 | 5.1 kb, ori-pAMα1-ori-ColE1 | Tcr | E. coli-S. aureus shuttle plasmid obtained from A. Wada (17) |
| pPG76 | 5-kb PstI (argI orf1–orf2–glmM) fragment from BB270 cloned in pAW8 | Tcr | This study |
a
Abbreviations: Em, erythromycin; femD is a synonym for glmM; hmrD1, suppressor mutation leading to high Mcr in glmM (femD) mutants; Mc, methicillin; Tc, tetracycline; r, resistant; s, susceptible.