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. 2022 Mar 9;20:28. doi: 10.1186/s12964-022-00835-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — ucMSC-Evs carry miR-378 to negatively regulate PSMD14. A a heatmap for the top 30 DE miRNAs in rat renal tissues and rMCs before and after 40 μg/mL Evs treatment according to a miRNA microarray analysis; B selection of the shared DE miRNAs in tissues and cells; C-D, expression of 7 upregulated miRNAs in renal tissues (C) and rMCs (D) after ucMSC-Evs treatment determined by RT-qPCR; E a functional classification analysis among the target mRNAs of miR-378 performed on the Panther System; F–G mRNA (F) and protein (G) expression of PSMD14 in rat renal tissues determined by RT-qPCR and western blot analysis, respectively; H, I mRNA (H) and protein (I) expression of PSMD14 in rMCs determined by RT-qPCR and western blot analysis, respectively; J binding relationship between miR-378 and PSMD14 determined using the luciferase reporter gene assay. In animal models, n = 6 in each group. Data were expressed as mean ± SD from at least three independent experiments. Data were analyzed by one- or two-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01 versus sham group or PBS group; ##p < 0.01versus PDGF-BB group or 0 μg/mL group; @@ p < 0.01 versus mimic control