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. 2022 Mar 9;20:28. doi: 10.1186/s12964-022-00835-1

Fig. 8.

Fig. 8

Overexpression of PSMD14 blocks the protective roles of ucMSC-Evs against PDGF-induced proliferation of rMCs. A, B mRNA and protein expression PSMD14 in cells after oe-PSMD14 transfection determined by RT-qPCR (A) and western blot analysis (B), respectively; C determination of the ubiquitination level of TGFBR1; D expression of TGF-β1 and phosphorylation of Smad2/3 in rMCs determined by western blot analysis; E proliferation of rMCs determined by EdU labeling; F viability of cells determined using a CTG kit; G ratio of live to dead cells confirmed by AO/EB double fluorescence staining; H apoptosis of cells determined by Hoechst 33258 staining; I apoptosis rate of cells determined by flow cytometry; J levels of the apoptosis-related proteins determined by western blot analysis. Data were expressed as mean ± SD from at least three independent experiments. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001 versus ucEvs + oe-NC group