Skip to main content
. 2022 Mar 7;10(3):e004054. doi: 10.1136/jitc-2021-004054

Figure 4.

Figure 4

BR105 does not inhibit T cell activation and proliferation. (A) Binding of indicated mAbs to SIRPγ was determined by ELISA. Data represent mean±SEM; representative of n=3 is shown. (B) PBMCs were cultured with 10 µg/mL of indicated mAbs in OKT3-coated plates for 3 days. Proliferation measured by CellTiter-Glo was normalized to control (PBMCs without addition of mAbs). (C) Dendritic cells and allogeneic PBMCs from three different donors were cultured 5 days with indicated mAbs at 10 µg/mL. Interferon γ secretion was quantified by ELISA and normalized to control (Dendritic cells and PBMCs without addition of mAbs). Data represent mean ± SEM; representative of n=3 donor is shown. *Indicate statistical differences compared with the respective isotype control group: *p<0.05, ***p<0.001, ****p<0.0001. ns, not significant.