TABLE 3.
Mutations in the Brazilian protease gene sequences related to PI resistance
| Sequence | Amino acid position related to PI resistancea
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U.S. consensus | 10L | 20K | 30D | 36M | 46M | 48G | 54I | 63P | 71A | 77I | 82V | 84I | 88N | 90L |
| Brazil F mutationb | I(5.8) | R(5.8) | I(76) | L(53) | M(5.8) | |||||||||
| V(5.8) | S(12) | |||||||||||||
| Total | 5.8 | 5.8 | 81.8 | 65 | 5.8 | |||||||||
| Brazil B mutationb | I(6.8) | R(2.2) | I(18) | V(2.2) | E(2.2) | L(2.2) | T(19) | T(4.5) | I(25) | A(2.2) | I(2.2) | V(4.5) | ||
| F(6.8) | M(2.2) | A(1) | V(2.2) | M(4.5) | ||||||||||
| S(1) | ||||||||||||||
| Total | 13.6 | 4.4 | 18 | 2.2 | 2.2 | 2.2 | 21 | 4.4 | 9 | |||||
a
Critical sites for resistance to particular drugs are as follows: nelfinavir, 30D and 88N; ritonavir and indinavir, 82V and 90L; saquinavir, 90L. Data are the amino acid changes and the percentages (in parentheses) of the isolates showing the particular mutations.
b
Variation in critical sites in Brazilian isolates belonging to subtypes F (n = 17) and B (n = 44).