Fig. 2.

Effects of miR-5195-3p overexpression on PCa cell proliferation and cell cycle progression. A MiR-5195-3p expression in PCa cell lines (PC-3, 22RV1, DU145, and LNCaP) and a nontransformed but immortalized prostate cell line RWPE-1 was measured by quantitative real-time PCR. B PC-3 and DU145 cells were transfected with the miR-5195-3p mimics or miR-NC. MiR-5195-3p expression in PC-3 and DU145 cells was detected by quantitative real-time PCR. C–D Cell proliferation was tested with CCK-8. MiR-5195-3p overexpression significantly inhibited the proliferation of PC-3 and DU145 cells. E–F Cell cycle distribution was determined by PI staining and flow cytometry analysis in PC-3 and DU145 cells. Data are presented as mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001 compared with miR-NC group. PI propidium iodide; NC negative control