Fig. 3.

CCNL1 was a direct target of miR-5195-3p in PCa. A The sequences of the putative miR-5195-3p binding sites in the wild-type and mutant CCNL1 3′-UTR. B–C Luciferase reporter plasmids carrying the CCNL1 wild-type 3′-UTR (CCNL1-WT) or CCNL1 mutant 3′-UTR (CCNL1-MUT) were transfected into PC-3 and DU145 cells with miR-5195-3p mimics or miR-NC. MiR-5195-3p upregulation suppressed luciferase activity of the wild-type but not the mutant 3′-UTR of CCNL1. Renilla luciferase activity was used as a control. D mRNA and E protein expression levels of CCNL1 following miR-5195-3p mimics or miR-NC transfection. F CCNL1 mRNA expression levels in 69 pairs of human PCa and matched adjacent normal tissues were measured by quantitative real-time PCR. Data are presented as mean ± standard deviation. **p < 0.01, ***p < 0.001 compared with miR-NC group. G MiR-5195-3p expression was inversely correlated with CCNL1 miRNA expression in PCa tissues, as demonstrated by the Spearman’s correlation coefficient