Table 1.

Primers used in this study to amplify Angiostrongylus spp. DNA

Primer name	F/R	Primer sequence	Position	No. of Angiostrongylus cox 1 mismatches
				A. mackerrasae	A. cantonensis
cox1F [S0962]	F	TTTGTTTTGATTTTTTGGTC	720–739	1	0
AngiCOI_forward [S0963]a	F	TTTTTTGGGCATCCTGAGGTTTAT	730–753	2	1
LCO1490	F	GGTCAACAAATCATAAAGATATTGG	47–71	8	9
AC1F [S0964]	F	CGGGTAAGAAGGAGGTTTTTG	806–826	0	2
AC2F [S0965]	F	AGTTATTGCGGTTCCTACGG	951–971	0	1
AC1R [S0966]	R	CCTTCACTCCCGTAGGAACC	960–979	0	2
AC2R [ S0967]	R	TTAGACAACATAACCCCAGTCAA	1078–1100	1	2
HCO2198	R	TAAACTTCAGGGTGACCAAAAAATCA	727–752	1	2
COI_Rb	R	TAAAGAAAGAACATAATGAAAATG	1147–1170	6	6
cox1R	R	AGGATAAATCTAAATACTTACGAGGA	1329–1354	7	6
AngiCOI_reverse	R	CGAGGATAACCATGTAAACCAGC	1312–1334	2	2

Note: Sequences of forward (F) and reverse (R) cox1 primers used in previous studies and their position relative to reference sequences A. cantonensis (GenBank: AMK570631) and A. mackerrasae (GenBank: MN793157) are shown (Apichat et al., 2016; Červená et al., 2019; Eamsobhana et al., 2017; Monte et al., 2012; Moreira et al., 2013; Nakaya et al., 2013; Okano et al., 2014; Rodpai et al., 2016; Tokiwa et al., 2012; Valentyne et al., 2020).

^aAlso named ‘COI_F’, ‘CO1_R’, ‘239’, ‘2575’.

^bAlso named ‘CO1_F’, ‘240’, ‘3021’.