. 2021 Aug 9;1:100046. doi: 10.1016/j.crpvbd.2021.100046

Table 4.

Accuracy and precision of various faecal egg counting techniques used for equine faecal samples

Methods used	Spiked epg/flotation solution	Parasite detected	Accuracy (%)	Precision (%)	Reference
Remodified McMaster (Sheatherʼs sugar)	Not applicable	Ascarids	6,387^a	NA	McQueary (1976)
Remodified McMaster (Sheatherʼs sugar)		Strongyles	1,403^a
Direct centrifugal flotation (Sheatherʼs sugar)		Ascarids	90^b
Direct centrifugal flotation (Sheatherʼs sugar)		Strongyles	86^b
Remodified McMaster (sodium chloride)		Ascarids	7,413^a
Remodified McMaster (sodium chloride)		Strongyles	1,456^a
Direct centrifugal flotation (sodium chloride)		Ascarids	45^b
Direct centrifugal flotation (sodium chloride)		Strongyles	97^b
Sedimentation	1 epg	Anoplocephala perfoliata	0.6	NA	Williamson et al. (1998)
	2 epg		0.6
	10 epg		0
	20 epg		2.4
	100 epg		3
	200 epg		12
Flotation 1	1 epg		0
	2 epg		0.18
	10 epg		0.2
	20 epg		0.7
	100 epg		1.9
	200 epg		3.9
Flotation 2	1 epg		0.6
	2 epg		1
	10 epg		2.2
	20 epg		1.2
	100 epg		10.2
	200 epg		31.9
FECPAK and McMaster	Not applicable	Strongyles	NA	FECPAK had lower variance and thus higher precision	Presland et al. (2005)
Centrifugal flotation	% of eggs recovered using centrifugal flotation	Cyathostomins	100	NA	Bello and Allen (2009)
McMaster	% of eggs recovered using centrifugal flotation	Cyathostomins	68–81	NA	Bello and Allen (2009)
McMaster	CV associated with the level of faecal pile, faecal bolus and sample from a faecal bolus, as well as the McMaster procedure	Cyathostomins	NA	CV more dependent on individual animal and higher than faecal bolus and McMaster CV	Denwood et al. (2012)
Combined zinc sulfate sedimentation-flotation	1 epg	Cyathostomins & A. perfoliata	1.20 & 0	NA	Becker et al. (2016)
	5 epg		2.12 & 1.60
	10 epg		3.46 & 1.32
	20 epg		2.00 & 0.80
	40 epg		4.13 & 0.90
	60 epg		2.22 & 0.91
	80 epg		3.51 & 1.00
McMaster	1 epg		0 & 0
	30 epg		22.20 & 22.20
	50 epg		44.40 & 26.60
	80 epg		61.00 & 30.50
	100 epg		37.70 & 39.90
	500 epg		75.90 & 14.90
	1000 epg		40.70 & NA
Smartphone prototype (accuracy: mean % epg; precision: 100–CV)^c	5 epg	Strongyles	18.91	71.6	Scare et al. (2017)
	50 epg		57.02
	500 epg		25.02
	1000 epg		29.15
McMaster (accuracy: mean % epg; precision: 100–CV)^c	5 epg		Not applicable	49.12
	50 epg		11.11
	500 epg		32.22
	1000 epg		21.67
Mini-FLOTAC (accuracy: mean % epg; precision: 100–CV)^c	5 epg		22.22	64.34
	50 epg		75.56
	500 epg		72.33
	1000 epg		87.94
McMaster (accuracy: mean % epg; precision: 100–CV)^c	5 epg	Strongyles	0	53.7	Noel et al. (2017)
	50 epg		16.67
	500 epg		43.33
	1000 epg		34.16
Mini-FLOTAC (accuracy: mean % epg; precision: 100–CV)^c	5 epg		33.33	83.24
	50 epg		28.33
	500 epg		52.33
	1000 epg		56.25
Mini-FLOTAC (accuracy: mean % epg; precision: % CV)^c	10 epg	Strongyles	90	49.6	Bosco et al. (2018)
	50 epg		90	10.9
	200 epg		96	8.1
	500 epg		82	3.1
McMaster (chamber) (accuracy: mean % epg; precision: % CV)^c	10 epg	Strongyles	70	135.6
	50 epg		78	51.4
	200 epg		84	23.1
	500 epg		92	10.9
McMaster (grid) (accuracy: mean % epg; precision: % CV)^c	10 epg	Strongyles	80	248.6
	50 epg		98	90.5
	200 epg		90	39.9
	500 epg		98	17.3
Cornell–Wisconsin (accuracy: mean % epg; precision: % CV)^c	10 epg	Strongyles	40	33.4
	50 epg		38	16.6
	200 epg		52	51.8
	500 epg		50	5.2
McMaster (precision as variance)^c	including FEC < 50 epg	Cyathostomins	NA	0.71	Britt et al. (2017)
McMaster (precision as variance)^c	excluding FEC < 50 epg			0.72
Mini-FLOTAC (precision as variance)^c	including FEC < 50 epg			0.52
Mini-FLOTAC (precision as variance)^c	excluding FEC < 50 epg			0.34
Modified-Wisconsin (precision as variability)^c	Not applicable	Strongyles	NA	0.045	Paras et al. (2018)
Three-chambered McMaster (precision as variability)^c				0.311
Mini-FLOTAC (precision as variability)^c				0.143
McMaster (precision as CV)^c	Not applicable	Strongylid	3rd highest egg count	0.45	Went et al. (2018)
Mini-FLOTAC (precision as CV)^c			2nd highest egg count	0.23
McMaster with Fill-Flotac (precision as CV)^c			highest egg count	0.45
Mini-FLOTAC with tongue depressor and cup (precision as CV)^c			4th highest egg count	0.20
Simple McMaster (accuracy: mean % epg; precision: % CV for spiked and natural infections, respectively)^c	Not applicable	Ascarids	65.53	62.95 & 31.20	Napravnikova et al. (2019)
		Strongyles	97.53	44.33 & 39.53
Concentration McMaster (accuracy: mean % epg; precision: % CV for spiked and natural infections, respectively)^c		Ascarids	83.18	35.71 & 17.92
		Strongyles	88.39	35.64 & 25.19
Mini-FLOTAC (accuracy: mean % epg; precision: % CV for spiked and natural infections, respectively)^c		Ascarids	90.28	18.95 & 14.51
		Strongyles	74.18	18.25 & 8.64
Simple flotation (precision as % CV)^c	Salt	Strongyles	NA	43.15	Silva et al. (2019)
Simple flotation (precision as % CV)^c	Sugar			52.43
Centrifuged flotation (precision as % CV)^c	Salt			68.97
Centrifuged flotation (precision as % CV)^c	Sugar			86.07
McMaster (precision as % CV)^c	Salt			95.75
McMaster (precision as % CV)^c	Sugar			53.96
Mini-FLOTAC (precision as % CV)^c	Salt			98.16
Mini-FLOTAC (precision as % CV)^c	Sugar			50.23
FECPAK^G1	Not applicable	Strongyles	100	NA	Tyson et al. (2020)
FECPAK^G2 (accuracy as a percentage of mean FECPAK^G1 egg count)^c	Not applicable	Strongyles	101	NA	Tyson et al. (2020)

Abbreviations: CV, coefficient of variation; epg, eggs per gram; NA, not assessed.

Mean epg.

Percent eggs recovered on four successive coverslips based on McMaster counts.

Measure of each of the corresponding parameter given in the published article.