. 2021 Nov 11;1:100059. doi: 10.1016/j.crpvbd.2021.100059

Table 1.

Oligonucleotide primers used in this study

Organism	Target gene	Oligonucleotide primer	Primer sequence (5′-3′)	Amplicon size (bp)	Reference
B. burgdorferi (s.l.)	flaB	Outer primer F	CTGCTGGCATGGGAGTTTCT	725	Yu et al. (2016)
		Outer primer R	TCAATTGCATACTCAGTACT	725	Yu et al. (2016)
		Nested primer F	GCAGTTCAATCAGGTAACGGC	411	Yu et al. (2016)
		Nested primer R	AGAAGGTGCTGTAGCAGGTG	411	Yu et al. (2016)
	rrf (5s)-rrl (23s)	23SN1	ACCATAGACTCTTATTACTTTGAC	373	Choi et al. (2007)
		23SC1	TAAGCTGACTAATACTAATTACCC	373	Choi et al. (2007)
		23SN2	ACCATAGACTCTTATTACTTTGACCA	227	Choi et al. (2007)
		5SCB	GAGAGTAGGTTATTGCCAGGG	227	Choi et al. (2007)
Rickettsia spp.	gltA	CS-78	GCAAGTATCGGTGAGGATGTAAT	401	Labruna et al. (2004)
	gltA	CS-323	GCTTCCTTAAAATTCAATAAATCAGGAT	401	Labruna et al. (2004)
	16S rRNA	Rick_16S_F3	ATCAGTACGGAATAACTTTTA	1328	Anstead & Chilton (2013)
		Rick_16S_F4	TGCCTCTTGCGTTAGCTCAC	1328	Anstead & Chilton (2013)
		rrs2_seq_1	AGGCCTTCATCACTCACTCG^a		Thu et al. (2019)
		rrs2_seq_2	CTACACGCGTGCTACAATGG^a		Thu et al. (2019)
		R16S_Fw1	AGAAAAAGCCCCGGCTAACTC^a		This study
		R16S_Rv1	CCATGCAACACCTGTGTGTG^a		This study
	ompB	120_2788	AAACAATAATCAAGGTACTGT	816	Roux & Raoult (2000)
	ompB	120_3599	TACTTCCGGTTACAGCAAAGT	816	Roux & Raoult (2000)
	sca4	D1f	ATGAGTAAAGACGGTAACCT	928	Sekeyova et al. (2001)
	sca4	D928r	AAGCTATTGCGTCATCTCCG	928	Sekeyova et al. (2001)

Primers that were used only for the sequencing of amplicons.