Table 3.
Results of the BLAST analysis of the Rickettsia spp. gltA, 16S rRNA, ompB, and sca4 sequences obtained in this study
| RG No. | Tick ID | Tick species and developmental stage | % Identity with the closest Rickettsia species (GenBank ID) |
|||
|---|---|---|---|---|---|---|
| gltA | 16S rRNA | ompB | sca4 | |||
| RG1 (n = 50) | Ipers20170019 | I. persulcatus (male) | 100% with R. helvetica (KU310588) | 100% with R. helvetica (MH618376) | 99% with R. helvetica (MF163037) | na |
| Ipers20170021 | I. persulcatus (female) | 100% with R. helvetica (KU310588) | 100% with R. helvetica (MH618376) | 99% with R. helvetica (MF163037) | na | |
| Ipers20170135 | I. persulcatus (nymph) | 100% with R. helvetica (KU310588) | 100% with R. helvetica (MH618376) | 99% with R. helvetica (MF163037) | na | |
| Ipers2017 Shikaoi L001 | I. persulcatus (larva) | 100% with R. helvetica (KU310588) | 100% with R. helvetica (MH618376) | 99% with R. helvetica (MF163037) | na | |
| Hmega2017 Hiroo L007 | H. megaspinosa (larva) | 100% with R. helvetica (KU310588) | 98% with R. raoultii (MK304546) 98% with R. conorii (NR_074480) 98% with R. helvetica (MH618376) |
na | na | |
| RG2 (n = 28) | Ipers20170103 | I. persulcatus (male) | 100% with “Ca. R. tarasevichiae” (MN450397) | 100% with “Ca. R. tarasevichiae” (MN446745) | na | na |
| Ipers20170025 | I. persulcatus (female) | 100% with “Ca. R. tarasevichiae” (MN450397) | 100% with “Ca. R. tarasevichiae” (MN446745) | na | na | |
| Ipers20170184 | I. persulcatus (nymph) | 100% with “Ca. R. tarasevichiae” (MN450397) | 100% with “Ca. R. tarasevichiae” (MN446745) | na | na | |
| Ipers2017 Taiki L001 | I. persulcatus (larva) | 100% with “Ca. R. tarasevichiae” (MN450397) | 100% with “Ca. R. tarasevichiae” (MN446745) | na | na | |
| RG3 (n = 7) | Hmega20170010a | H. megaspinosa (male) | 100% with “Ca. R. principis” (AY578115) | 99% with R. raoultii (MK304546) 99% R. conorii (NR_074480) |
100% with “Ca. R. principis” (MG544987)b | 99% with R. heilongjiangensis (AP019865) |
| Hmega20170004a | H. megaspinosa (female) | 100% with “Ca. R. principis” (AY578115) | 99% with R. raoultii (MK304546) 99% R. conorii (NR_074480) |
100% with “Ca. R. principis” (MG544987)b | 98% with R. heilongjiangensis (AP019865) | |
| Hmega2017 Hiroo L009a | H. megaspinosa (larva) | 100% with “Ca. R. principis” (AY578115) | 99% with R. raoultii (MK304546) 99% with R. conorii (NR_074480) |
100% with “Ca. R. principis” (MG544987)b | 98% with R. heilongjiangensis (AP019865) | |
| RG4 (n = 1) | Ipers20170166 | I. persulcatus (male) | 99% with “Ca. R. tarasevichiae” (MN450397) 89% with R. helvetica (KU310588) |
100% with R. helvetica (MH618376) | 99% with R. helvetica (MF163037) | na |
Abbreviations: Ca., Candidatus; na, not amplified; RG, gltA genotype of Rickettsia spp.
a
Identities with “Ca. R. principis” 16S rRNA and sca4 are not shown due to absence of reference sequences in the database.
b
Query coverage was 95%.