. 2021 Jun 3;1:100034. doi: 10.1016/j.crpvbd.2021.100034

Table 1.

List of the PCR primers used in the present study

Targeted microorganism	Gene	Primer name	Sequence	Length (bp)	Reference
Tick (mitochondrial)	16S rRNA	16S + 1	CCGGTCTGAACTCAGATCAAG	460	Mangold et al. (1998)
Tick (mitochondrial)	16S rRNA	16S-1	GCTCAATGATTTTTTAAATTGCTG	460	Mangold et al. (1998)
Rickettsia sp.	gltA	CS-78	GCAAGTATCGGTGAGGATGTAAT	401	Labruna et al. (2004)
Rickettsia sp.	gltA	CS323	GCTTCCTTAAAATTCAATAAATCAGGAT	401	Labruna et al. (2004)
Rickettsia sp.	gltA	CS-239	GCTCTTCTCATCCTATGGCTATTAT	834	Labruna et al. (2004)
Rickettsia sp.	gltA	CS-1069	CAGGGTCTTCGTGCATTTCTT	834	Labruna et al. (2004)
Rickettsia spotted fever group	ompA	Rr190.70p	ATGGCGAATATTTCTCCAAAA	532	Roux et al. (1996)
		Rr190.602n	AGTGCAGCATTCGCTCCCCCT	532
		ompB-OF	GTAACCGGAAGTAATCGTTTCGTAA	511
Rickettsia sp.	ompB	ompB-O	GCTTTATAACCAGCTAAACCACC		Choi et al. (2005)
		ompB SFG IF	GTTTAATACGTGCTGCTAACCAA	420
		ompB SFG IR	GGTTTGGCCCATATACCATAAG
Anaplasmataceae	16S rRNA	EHR16SD	AGAGTTTGATCCTGGCTCAG	1500	Inokuma et al. (2001)
Anaplasmataceae	16S rRNA	EHR16SR	ACGGCTACCTTGTTACGACTT	1500	Inokuma et al. (2001)
Ehrlichia spp.	Dsb	Dsb-330	GATGATGTTTGAAGATATSAAACAAAT	409	Doyle et al. (2005)
		Dsb-720	CTATTTTACTTCTTAAAGTTGATAWATC
		Dsb-380	ATTTTTAGRGATTTTCCAATACTTGG
Ehrlichia spp.	groEl	HS1a	AITGGGCTGGTAITGAAAT	1297	Lotric-Furlan et al. (1998)
		HS6a	CCICCIGGIACIAIACCTTC
		HS43	ATWGCWAARGAAGCATAGTC
		HSVR	CTCAACAGCAGCTCTAGTAGC
Borrelia spp.	16S rRNA	LoneTop	CTGGCAGTGCGTCTTAAGCA	869	Cyr et al. (2005)
Borrelia spp.	16S rRNA	Tec1a/p	TCTTGCGAGCATACTCCCCAG	869	Cyr et al. (2005)
Borrelia spp.	flab	Fla LL	ACATATTCAGATGCAGACAGAGGT	665	Barbour et al. (1996)
		Fla RL	GCAATCATAGCCATTGCAGATTGT
		FlaRS	CTTTGATCACTTTCATTCTAATAGC
		FlaLS	AACAGCTGAAGAGCTTGGAAT
Borrelia spp.	Flab	Fla LS	AACAGCTGAAGAGCTTGGAATG	354	Barbour et al. (1996)
Borrelia spp.	Flab	Fla RS	CTTTGATCACTTATCATTCTAATAGC	354	Barbour et al. (1996)
Piroplasmid	18S rRNA	BAB 143-167	CCGTGCTAATTGTAGGGCTAATACA	551	Soares et al. (2017)
Piroplasmid		BAB 694-667	GCTTGAAACACTCTARTTTTCTCAAAG	551	Soares et al. (2017)