Fig. 2 |. neoT_reg cell reduction causes outgrowth of an Il13ra1⁺ fibroblast population in skin fascia.

a–d, scRNA-seq of Lin⁻ stromal cells (EpCAM⁻CD31⁻CD45⁻) sorted from skin of control and ΔneoT_reg mice at P22. Il13ra1⁺ fibroblast (FB) clusters are circled. n = 2 animals pooled per group. prolif, proliferative. a, Unsupervised clustering. b, Relative cluster abundance in ΔneoT_reg skin. Arrows indicate Il13ra1⁺ FB clusters. c, Gene expression by cluster in control sample. d, Gating strategy for Il13ra1⁺ fibroblasts in P25 control skin. The top plot shows total live cells. e, f, Expression of selected Il13ra1⁺ FB markers identified by scRNA-seq (e) was measured by quantitative PCR of sorted cells (f) to validate the gating strategy in d (bulk SCA1⁺: CD34⁺ SCA1⁺CD26^+/−CD9⁺; other stroma: CD34⁻SCA1⁻; f). n = 4 animals. g, Localization of Il13ra1⁺ fibroblasts stained for CD26 in back skin of P25 mice. Scale bar, 50 μm. h, Flow cytometry quantification of Il13ra1⁺ fibroblasts at P22. n = 12 animals. Data are displayed as the mean ± s.d. from one independent experiment, representative of one (a–c, e) or two-to-three repeats (d, f–h). *P < 0.05, **P < 0.01 (all two-sided); repeated measures ANOVA with Geisser–Greenhouse correction and Dunnett’s multiple comparisons test (f) or Student’s t-test (h).