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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Nat Struct Mol Biol. 2022 Feb 14;29(2):162–171. doi: 10.1038/s41594-022-00723-9

Extended Data Fig. 10 |. A2062 adopts a lumen conformation in the RZD-stalled complex with a Cfr-modified ribosome.

Extended Data Fig. 10 |

(a) A2062 adopts both the lumen and the rotated, tunnel wall conformation in the RZD-only bound ribosome. In the rotated wall conformation, A2062 engages in H-bonding interactions with A2503 and RZD. (b) A2062 is only observed in the rotated conformation to H-bond with A2503 and RZD in the WT stalled complex. (c) A2062 is observed in the lumen conformation in the RZD-stalled complex with a Cfr-modified ribosome. Coulomb potential density is contoured at 4.0s in surface representation and 1.0s in mesh representation from unsharpened cryo-EM density maps.