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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Nat Struct Mol Biol. 2022 Feb 14;29(2):162–171. doi: 10.1038/s41594-022-00723-9

Fig. 2 |. Cryo-EM structures of LZD- and RZD-stalled ribosome complexes.

Fig. 2 |

a, Stalled complexes were generated by performing coupled in vitro transcription–translation reactions in the presence of the oxazolidinone (Oxa) antibiotic LZD or RZD. Complexes were further purified by sucrose gradient fractionation. b, Cross-section of the cryo-EM density map of the stalled 70S ribosome in complex with peptidyl-tRNA and oxazolidinone. Insets: close-up views of LZD or RZD in complex with the MFKAF nascent peptide. Coulomb potential density is contoured at 4.0σ in the surface representation and 1.0σ in the mesh representation. The figure was prepared using unsharpened cryo-EM density maps.