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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Nat Struct Mol Biol. 2022 Feb 14;29(2):162–171. doi: 10.1038/s41594-022-00723-9

Fig. 3 |. Nascent peptide with penultimate alanine stabilizes oxazolidinone binding to the ribosome.

Fig. 3 |

The Coulomb potential density is contoured at 4.0σ in the surface representation and 1.0σ in the mesh representation from unsharpened cryo-EM density maps. a, Comparison of LZD density in the LZD-only bound (LZD-70S) and the LZD-stalled complex (LZD-SRC). b, Comparison of RZD density in the RZD-only bound (RZD-70S) and the RZD-stalled complex (RZD-SRC). c, Close-up view of the CH–π interaction between the B-ring of LZD and the penultimate alanine (Ala4). d, Close-up view of the CH–π interaction between the RZD B-ring and the penultimate alanine. e,f, Schematics of the CH–π interaction involved in stabilizing antibiotic binding in LZD-stalled (e) and RZD-stalled (f) ribosome complexes. g, Density of the exit-tunnel rRNA nucleotide A2062 in the LZD-only bound structure, highlighting two conformations. h, Density of A2062 in the LZD-stalled structure, with only one observed conformation.