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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Nat Struct Mol Biol. 2022 Feb 14;29(2):162–171. doi: 10.1038/s41594-022-00723-9

Extended Data Fig. 1 |. Processing workflow, classification tree, FSC curves, and local resolution estimation for each map.

Extended Data Fig. 1 |

Micrographs were CTF corrected and curated for ice quality, followed by unsupervised particle picking. 2D classification was used to remove residual ice particles, with all others subjected to multi-class 3D classification and refinement approaches to remove particles without tRNA (for SRCs) and select for good particles. Final FSC curves are presented along with center-slab representation of local resolution. All steps were carried out within the cisTEM (v1.0.0-beta) framework with the exception of local resolution estimation, which used the Relion (v3.1.2) implementation.