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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Mol Cell Endocrinol. 2021 Feb 13;527:111208. doi: 10.1016/j.mce.2021.111208

Fig. 2. Kiss1 and Kiss1r expression in rat ovary.

Fig. 2.

Oocytes and granulosa cells (GCs) were isolated from the ovaries collected 4h after hCG administration to PMSG treated 4-wk-old rats. RT-qPCR analyses showed that expression of Kiss1 was remarkably higher in (GCs) (A), whereas Kiss1r in the oocytes (B). RNA sequencing analyses showed a significantly higher expression of Kiss1 in wildtype (WT) GCs compared to Erβnull GCs (C), but a similar level of Kiss1r expression in both WT and Erβnull oocytes (E). RT-qPCR data from GCs and oocytes further confirmed the results of RNA-sequencing (D and F). RNA-sequencing data are presented in triplicates. RT-qPCR data are represented as mean ± SE. n = 6. *P ≤ 0.05.