FIG 6.
The P. aeruginosa expressing Gp21 gains the ability to defend against phage attacks. (A) Spot assay was used to evaluate phage resistance. Three microliters each of serial 10-fold dilution (106 to 104) of phage vB_Pae_QDWS was spotted onto PAO1-pBBR5, PAO1-gp21, and PaΔpilB-pBBR5 for assays. (B) Antiphage activity assays of PAO1-pBBR5, PAO1-gp21, and PaΔpilB-pBBR5. Growth curves of P. aeruginosa strains infected with phage vB_Pae_QDWS at an MOI of 0.1 in a 96-well microtiter plate containing 200-μL cultures were detected by a multimode reader. The values were the averages of three measures with standard deviation.