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. 2022 Mar 9;96(5):e02057-21. doi: 10.1128/jvi.02057-21

FIG 1.

FIG 1

Circulating HSV-1 gD-specific memory B cell profile in asymptomatic and symptomatic herpes-infected individuals. PBMCs from asymptomatic and symptomatic HSV-1-infected individuals were stained for HSV-1 gD antigen-specific memory B cells. PBMCs were also treated with IL-2 and resiquimod for 5 days for polyclonal stimulation of memory B cells in plasma cells (antibody secreting cells [ASC]). The stimulated cells were then analyzed for HSV-1 gD-specific IgG/IgA ASC and total IgG/IgA ASC by ELISPOT. (A) FACS plot showing the gating strategy for HSV-1-specific memory B cells (CD19+CD27+ B cells) in PBMCs of asymptomatic (ASYMP) (Left) and symptomatic (SYMP) (Right) HSV-1-infected individuals. (B) Graph showing percentage of HSV-1-specific memory B cells (CD19+CD27+ B cells) in PBMCs of ASYMP and SYMP HSV-1-infected individuals. (C) Representative ELISPOT images for anti-HSV-1 IgG ASC (Left, top) and anti-HSV-1 IgA ASC (Left, bottom) from PBMCs of ASYMP and SYMP HSV-1-infected individuals and HSV-1 uninfected individuals (polyclonally stimulated for maturation of memory B cells to ASC). (D) Graph showing anti-HSV-1 IgG (Left, top) and IgA (Left, bottom) ASC; total IgG (Right, top) and total IgA (Right, bottom) ASC from PBMCs of ASYMP and SYMP HSV-1-infected individuals and HSV-1 uninfected individuals. Statistical analysis was done using Student's t test. NS, not significant.