FIG 2.

Correlation of HSV-1-specific memory B cell and memory T follicular helper T cells in herpes-infected individuals. PBMCs from asymptomatic and symptomatic HSV-1-infected individuals were stained for switched memory B cells (CD19⁺CD27⁺IgD⁻) and HSV-1 gD-specific switched memory B cells. (A) FACS plot showing gating strategy for HSV-1-specific switched memory B cells (CD19⁺CD27⁺ B cells) in PBMCs of ASYMP (Left) and SYMP (Right) HSV-1-infected individuals. (B) Graph showing percentage of HSV-1-specific switched memory B cells (CD19⁺CD27⁺IgD⁻ B cells) in PBMC of ASYMP and SYMP HSV-1-infected individuals. (C) FACS plot showing gating strategy for T follicular helper cells (T_fh) (CD3⁺CD4⁺CXCR5⁺ T cells) and T follicular helper memory cells (T_fh memory) (CD3⁺CD4⁺CD45R0⁺CXCR5⁺ T cells) in PBMC of ASYMP (Left) and SYMP (Right) HSV-1-infected individuals. (B) Graph showing correlation of the percentage of HSV-1-specific switched memory B cells (CD19⁺CD27⁺IgD⁻ B cells) and T follicular helper memory cells (T_fh memory) (CD3⁺CD4⁺CD45R0⁺CXCR5⁺ T cells) in PBMCs of HSV-1-infected individuals. Statistical analysis was done using Student's t test. NS, not significant.