FIG 6.

T follicular helper (T_fh) and T follicular helper memory (T_fh memory) cell profile in spleen of ASYMP and SYMP HSV-1-infected mice. B6 mice (n = 6) were infected with HSV-1 McKrae (1 × 10⁶ PFU/eye) by scarification, and at day 35 p.i., reactivation was done by 60 s corneal UV-B irradiation. Mice were categorized as ASYMP/SYMP and euthanized at day 6 postreactivation. Spleen cells were collected for flow cytometry staining of T_fh (CD3⁺CD4⁺CXCR5⁺PD-1⁺ cells) and T_fh memory (CD3⁺CD4⁺CD44⁺CXCR5⁺PD-1⁺ cells). (A) Representative FACS plots for T_fh cells (CD3⁺CD4⁺CXCR5⁺PD-1⁺ cells) are shown in the top panel, and T_fh memory cells (CD3⁺CD4⁺CD44⁺CXCR5⁺PD-1⁺ cells) are shown in bottom panels for ASYMP (Left) and SYMP (Right) infected mice. (A) Graph showing percentage of T_fh cells (top) and T_fh memory cells (bottom) in spleen of ASYMP and SYMP infected mice is shown.