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Expression of A/Viet Nam/1203/2004 H5N1 PA-X WT and mutants by immunofluorescence. Human HEK293T cells (24-well plate format, 2.5 × 105 cells/well, triplicates) were transiently transfected with 1 μg of the pCAGGS plasmids encoding PA-X WT or the indicated mutations. At 24 hpt, cells were fixed, permeabilized, and stained with an Ab against the HA epitope tag (green). Cell nuclei were stained with DAPI (blue). Representative images are shown. Scale bar, 10 μm.
